Sclerostin ELISA
Method :
Sandwich ELISA (HRP/TMB), 12 × 8-well detachable strips
Sample type :
Serum, EDTA plasma, citrate plasma, heparin plasma, urine, cell culture supernatant
Sample volume :
20 µl per well
Assay time :
18–24 h / 1 h / 30 min
Sensitivity :
3.2 pmol/L (≈ 72 pg/mL)
Standard range :
0 – 240 pmol/L (≈ 0 – 5 400 pg/mL)
Conversion factor :
1 pmol/L = 22.5 pg/mL (MW ~ 22.5 kDa)
Precision :
• Between-run (n = 6): ≤ 10% CV • Within-run (n = 8): ≤ 7% CV
Specificity :
Detects endogenous and recombinant human Sclerostin
Cross-reactivity :
No cross-reactivity observed with Wise (Sostdc1), Noggin, or rat/mouse Sclerostin
Use :
For research use only
size :
1 Kit
Sandwich ELISA (HRP/TMB), 12 × 8-well detachable strips
Sample type :
Serum, EDTA plasma, citrate plasma, heparin plasma, urine, cell culture supernatant
Sample volume :
20 µl per well
Assay time :
18–24 h / 1 h / 30 min
Sensitivity :
3.2 pmol/L (≈ 72 pg/mL)
Standard range :
0 – 240 pmol/L (≈ 0 – 5 400 pg/mL)
Conversion factor :
1 pmol/L = 22.5 pg/mL (MW ~ 22.5 kDa)
Precision :
• Between-run (n = 6): ≤ 10% CV • Within-run (n = 8): ≤ 7% CV
Specificity :
Detects endogenous and recombinant human Sclerostin
Cross-reactivity :
No cross-reactivity observed with Wise (Sostdc1), Noggin, or rat/mouse Sclerostin
Use :
For research use only
size :
1 Kit
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Internal Reference:
BM-BI-20492