FluoBolt WNT3A FIA

Method :
Metal-Enhanced Direct Sandwich Fluorescence Immunoassay, 96-well plate format
Sample type :
Serum, plasma
Sample volume :
20 µl per well
Assay time :
Overnight at room temperature (18–26°C)
Sensitivity :
LOD (0 pmol/L + 3 SD): 51 pmol/L
LLOQ: 175 pmol/L
Standard range :
0 – 2 800 pmol/L (≈ 0 – 110 320 pg/mL)
Conversion factor :
1 ng/mL = 25 pg/mL (MW ~ 39.4 kDa)
Detection range :
51 – 2 800 pmol/L (≈ 2 009 – 110 320 pg/mL)
Precision :
• Between-run (n = 4): ≤ 10% CV
 • Within-run (n = 4): ≤ 11% CV
Detection target :
Detects human endogenous and recombinant WNT3A
Cross-reactivity :
Human WNT3A shares ~100–97% sequence identity with primates, 96–95% with bears, 96% with whales and mice. Cross-reactivity with other species has not been tested
Use :
For research use only
size :
1 Kit