FluoBolt Noggin FIA

Method :
Metal-Enhanced Direct Sandwich Fluorescence Immunoassay, 96-well plate format
Sample type :
Serum, plasma
Sample volume :
20 µl per well
Assay time :
Overnight at room temperature (18–26°C) or 4 h at 37°C
Sensitivity :
LOD (0 pmol/L + 3 SD): 1 pmol/L
LLOQ: 5 pmol/L
Standard range :
0 – 250 pmol/L (≈ 0 – 16.7 ng/mL)
Conversion factor : 
1 ng/mL = 15 pmol/L (MW ~ 66 kDa)
Detection target :
Detects free, bioactive human NOGGIN not bound to BMPs. Signal reduction observed when equimolar amounts of BMP-2, -4, or -7 are added
Cross-reactivity :
Human NOGGIN shares 99%, 99%, 98%, 97%, and 89% sequence identity with mouse, rat, bovine, equine, and chicken NOGGIN, respectively. Cross-reactivity with other species has not been tested
Use :
For research use only
size :
1 Kit