FluoBolt Klotho FIA

Method :
Metal-Enhanced Direct Sandwich Fluorescence Immunoassay, 96-well plate format
Sample type :
Serum, plasma
Sample volume :
10 µl per well
Assay time :
Overnight at room temperature (18–26°C)
Sensitivity :
LOD (0 pmol/L + 3 SD): 2.5 pmol/L
LLOQ: 25 pmol/L
Standard range :
0 – 400 pmol/L (≈ 0 – 24 840 pg/mL)
Conversion factor :
1 ng/mL = 16 pmol/L (MW ~ 62.1 kDa)
Detection range :
2.5 – 400 pmol/L (≈ 155 – 24 840 pg/mL)
Precision :
• Between-run (n = 4): ≤ 10% CV 
• Within-run (n = 4): ≤ 11% CV
Detection target :
Specifically detects human endogenous and recombinant α-KLOTHO; no cross-reactivity with β-KLOTHO
Cross-reactivity :
Human α-KLOTHO shares ~98–97% sequence identity with higher apes, 95–91% with bovines, 91–89% with pinnipeds, and 87% with mice. Cross-reactivity with other species has not been tested
Use :
For research use only
size :
1 Kit